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Percentage of sperm with tail-associated protein tyrosine phosphorylation after incubation for 4 h in NCM, CM, CM—NaCl, CM—Na+, CM + 25 mM NH4Cl, CM at pH 7.9, CM + 1 mM caffeine, and CM + 2.5–5–10–25–50 mM procaine. Stallion sperm tail-associated protein tyrosine phosphorylation was induced by 10 mM procaine CM, medium pH 7.9, and caffeine CM. Values that differ significantly (P < 0.05) are indicated by different small letters.
