Figure 2.

Motility patterns indicative of hyperactivated motility were assessed by CASA in stallion sperm incubated for 0.5, 2, 4, and 6 h in CM (black bars), CM + 2 mM EGTA (grey bars), CM + 2.5 mM procaine (white bars), and CM + 2.5 mM procaine +2 mM EGTA (striped bars) at pH = 7.4 and pH = 7.9 (n = 3 samples in each group; three replicates). Parameters of interest were (A) ALH and (B) VCL. Hyperactivated motility of spermatozoa induced by 2.5 mM procaine is independent of extracellular Ca²⁺ and pH. For both ALH and VCL, values that differ (P < 0.05) are indicated by different small letters.