Figure 4.
Evaluation of different motility parameters ((A) percentage of total motile or TM; (B) percentage of progressively motile or PM; (C) ALH; and (D) VCL) during 6 h incubation of stallion spermatozoa in (1) CM, (2) CM + 2.5 mM procaine, (3) CM + 25 mM NH4Cl, and (4) CM pH 7.9 (n = 3 samples in each group; three replicates). Hyperactivated motility in sperm suspensions was triggered by exposure to 5 mM procaine and 25 mM NH4Cl and to a lesser extent by CM at pH 7.9. For percentage of progressively motile, ALH and VCL, values that differ (P < 0.05) are indicated by different small letters. Repeated measure ANOVA with Greenhouse-Heisser and Bonferroni correction Scheffé post hoc tests were performed for pairwise comparison.