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. 2019 Aug 9;101(5):1001–1017. doi: 10.1093/biolre/ioz139

Figure 5.

Figure 5

Effects of cell density on nuclear localization of phosphorylated YAP1 (Ser127) in bovine GCs. Granulosa cells were seeded overnight on coverslips in six-well dishes at increasing cell densities from 0.125 to 0.50 × 106 cells/well. (A) Representative micrograph of GC plated at 0.125 × 106 cells/well; phosphorylated Yes-associated protein (p-YAP1 (Ser127)) (a), YAP1 (b), colocalization of p-YAP1 (Ser127) and YAP1 (c), 20x magnification of colocalization of p-YAP1 (Ser127) and YAP1 (d), p-YAP1 (Ser127) (e), alpha-tubulin (f), colocalization of p-YAP1 (Ser127) and alpha-tubulin (g), 20× magnification of colocalization of p-YAP1 (Ser127) and alpha-tubulin (h), p-YAP1 (Ser127) (i), DAPI (j), colocalization of p-YAP1 (Ser127) and DAPI (k), 20× magnification of colocalization of p-YAP1 (Ser127) and alpha-tubulin (l). (B) Representative micrograph of GC plated at 0.25 × 106 cells/well. (C) Representative micrograph of GC plated at 0.50 × 106 cells/well. (D) Quantitative analysis of colocalization of p-YAP1 with YAP1, alpha-tubulin, and DAPI. Data are represented as means ± SEM, n = 3 experiments. **Significant difference as compared to 0.125 × 106 cells/well, P < 0.05. Micron bar represents 20 μm (63×) and 50 μm (20×).