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. 2019 Aug 9;101(5):1001–1017. doi: 10.1093/biolre/ioz139

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Published by Oxford University Press on behalf of Society for the Study of Reproduction 2019. This work is written by (a) US Government employee(s) and is in the public domain in the US.

This work is written by US Government employees and is in the public domain in the US.

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Effects of cell density on nuclear localization of TAZ in bovine GCs. Granulosa cells were seeded overnight on coverslips in 6-well dishes at increasing cell densities from 0.125–0.50 × 10⁶ cells/well. (A) Representative micrograph of GC plated at 0.125 × 10⁶ cells/well; WW domain-containing transcription regulator 1 (TAZ; WWTR1) (a), alpha-tubulin (b), colocalization of TAZ and alpha-tubulin (c), 20× magnification of colocalization of TAZ and alpha-tubulin (d), TAZ (e), DAPI (e), colocalization of TAZ and DAPI (g), 20× magnification of colocalization of TAZ and alpha-tubulin (h). (B) Representative micrograph of GC plated at 0.25 × 10⁶ cells/well. (C) Representative micrograph of GC plated at 0.50 × 10⁶ cells/well. (D) Quantitative analysis of colocalization of TAZ with alpha-tubulin and DAPI. Data are represented as means ± SEM, n = 3 experiments. ^**Significant difference as compared to 0.125 × 10⁶ cells/well, P < 0.05. Micron bar represents 20 μm (63×) and 50 μm (20×).