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. 2019 Aug 9;101(5):1001–1017. doi: 10.1093/biolre/ioz139

Figure 8.

Figure 8

Effects of siRNA-mediated knockdown of YAP1 and TAZ on TGFα-induced proliferation in GCs. YAP1 and TAZ mRNA were silenced using siYAP1 and siTAZ in bovine GCs. Following knockdown, GCs were treated with or without transforming growth factor-alpha (50 ng/mL; TGFα) to promote cell proliferation. (A) Representative western blot showing phospho-YAP1(Ser127)  and   YAP1   protein   expression  in  siGlo  (siCTL)  or  siYAP1 knockdown GCs, following treatment with or without TGFα. (B) Densitometric analyses of YAP1 protein expression obtained from siCTL (open bars) and siYAP1 (closed bars). Bars represent means ± SEM, n = 5. (C) Cell counts following knockdown of YAP1 cells and treatment with TGFα (closed bars) or without TGFα (open bars). Bars are means ± SEM, n = 3. (D) Representative western blot showing YAP1 and TAZ protein expression in GCs following 48 h treatment with siCTL, siYAP1, siTAZ, or a combination of siYAP1 and siTAZ (siY + siT). Beta-actin (ACTB; loading control). (E) Quantitative analysis showing DNA synthesis for siCTL, siYAP1, siTAZ, or siY + siT knockdown cells treated with control (open bars) or TGFα (black bars). Data are represented as means ± SEM (n = 3) of the average fold change from control in each experiment. **Significant differences between treatment groups, as compared to siCTL, P < 0.05.