Table 2.

Michaelis-Menten kinetic parameters calculated from Eadie-Hofstee plot analysis for transporter-mediated cellular uptake of FA and its derivatives 1-3 into primary astrocytes. The data are presented as the mean ± SD; n = 3.

Compound	Primary transport mechanism (LAT1)		Primary transport mechanism (OATP/OAT)
	V max (nmol/min/mg protein)	K m (μM)	V max (nmol/min/mg protein)	K m (μM)
FA-derivative 1	0.008 ± 0.002	4.1 ± 2.1	0.033 ± 0.402	101.1 ± 19.7
FA-derivative 2	0.013 ± 0.001	4.2 ± 0.8	0.172 ± 0.013	153.8 ± 15.0
FA-derivative 3	Autoactivation
FA	Autoactivation