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. 2019 Nov 13;2019:2343867. doi: 10.1155/2019/2343867

Figure 4.

Figure 4

IL-6 in ADSC-CM promoted flap recovery and increased the number of hair follicles. (a) The conditioned media were collected from ADSCs pretreated with or without a control immunoglobulin G (IgG), an IL-6-neutralizing antibody (anti-IL-6), an IL-6 siRNA transfection (si-IL-6), or a control scrambled oligonucleotide sequence (si-Scramble, si-Con) transfection. Photographs of I/R-induced flaps in the presence or absence of ADSC-CM, ADSC+IgG-CM (IgG), ADSC+anti-IL-6 antibody-CM (anti-IL-6), si-Scramble-ADSC-CM (si-Scramble), or si-IL-6-ADSC-CM (si-IL-6). The sections were stained with H&E. Bar = 1 mm. (b) Higher magnification of the area enclosed by the rectangles in (a). Bar = 500 μm. (c) Statistical analysis of hair follicles under HPF. n = 6 for each group. ∗p < 0.05 versus the I/R group; †p < 0.05 versus the CM group; ǂp < 0.05 versus the IgG or the si-Scramble group.