Figure 5.
(a) Representative immunoblot from the pull-down assay of mouse mesenteric arteries for active Rac1 (Rac1-GTP). (b) Immunoblot analysis for Nrf2. Cytoplasmic (Cyto) and nuclear (Nuc) fractions were prepared from untreated mouse mesenteric arteries or treated with BRP2. GAPDH and HDAC2 were used as cytoplasmic and nuclear markers, respectively. Right: nuclear/cytoplasmic ratios for Nrf2 are plotted from densitometry (n = 3). (c) Representative immunoblot for MnSOD in mouse mesenteric arteries treated with BRP2 (100 μM) and Ang II (10−5 M) (n = 3).