Figure 3.

NOX2 oxidase subunit expression in activated human macrophages. PDBu-differentiated THP-1 macrophages or M-CSF-differentiated human primary macrophages were left untreated (MΦ) or treated with IFN-γ+LPS (M1) or IL-4 (M2) for 3 to 72 hours. Time course of NOX2 (a, g), p47phox (b, h), and p67phox (c, i) mRNA expression determined by RT-qPCR and expressed relative to the average MΦ (untreated) value. Protein expression of NOX2 (d, j), p47phox (e, k), and p67phox (f, l) after 24 (primary macrophages) or 72 hours (THP-1 macrophages), determined via western blotting. Representative blots, depicting n = 3, are shown below each graph with GAPDH used as a loading control. All results presented as mean ± SEM, n = 5‐8. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. MΦ (1-way ANOVA followed by Dunnett's post hoc test).