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. 2019 Aug 28;100(4):234–243. doi: 10.1111/iep.12331

Figure 3.

Figure 3

The expression of migratory cranial neural crest cell and postmigratory cranial neural crest cell markers in control and sema3E morphants. A, The expression of migratory cranial neural crest cell markers, crestin and sox10, in control and sema3E morphants at 20 hpf. Black brackets indicate the expression of crestin or sox10 in the rhombomeres. Red arrows denote the scattered cranial neural crest cells. B, Quantitative PCR to quantify the expression of crestin and sox10 in the hindbrain in control and sema3E morphants. C, The expression of postmigratory cranial neural crest cell markers, dlx2a and hand2, in control and sema3E morphants at 23 hpf. Red arrows denote the reduced postmigratory cranial neural crest cell markers in sema3E morphants. D, Quantitative PCR to quantify the expression of crestin and sox10 in the hindbrain in control and sema3E morphants (mean ± SD, Student's t test, *P < 0.05, n = 3)