Figure 4.

Knockdown of sema3E resulted in impaired migration of cranial neural crest cells in sox10:EGFP transgenic embryos at 22 hpf. A, Dorsal view of the immunostained control MO‐injected sox10:EGFP transgenic embryos. B, Dorsal view of the immunostained sema3E MO‐injected sox10:EGFP transgenic embryos. Red arrows denote the misrouted GFP‐expressing cranial neural crest cells. C, Lateral view of the immunostained control MO‐injected sox10:EGFP transgenic embryos. D, Lateral view of the immunostained sema3E MO‐injected sox10:EGFP transgenic embryos. Red arrows denote the error‐migrated GFP‐expressing cranial neural crest cells, which are present in the dorsal part of sema3E MO‐injected sox10:EGFP transgenic embryos