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. 2019 Oct 7;181(4):1683–1703. doi: 10.1104/pp.19.00574

Figure 9.

Figure 9.

MtbHLH2 and MtCP77 play opposing roles in plant PCD and ROS accumulation. A and B, The accumulation of H2O2 in longitudinal sections of bhlh2 (A) or MtCP77OE nodules (B) at 28 dpi was detected. A higher concentration of H2O2 was found in the bhlh2 (A) or MtCP77OE nodules (B). Bars = 200 μm. C and D, DNA fragmentation in longitudinal sections of wild-type (WT) and bhlh2 nodules at 28 dpi (C) or wild-type and cp77 nodules at 49 dpi (D) was detected by TUNEL assay. DNA fragmentation resulting from the cell death signaling cascade is shown as brown signals in both plant nuclei and rhizobia of bhlh2 nodules at 28 dpi (C) or wild-type nodules at 49 dpi (D). Bars = 200 μm. E and F, Magnification of the boxed areas in (C) and (D). Bars = 50 μm.