Table 1. NADKc kinetic parameters.
Substrate Varied | Constant Substrate | Km | kcat | kcat/Km |
---|---|---|---|---|
µm | s−1 | µm−1 s−1 | ||
ATP | NAD+ (10 mm) | 203 ± 30 | 41 ± 2 | 0.2 |
CTP | NAD+ (10 mm) | 283 ± 70 | 42 ± 1 | 0.15 |
GTP | NAD+ (10 mm) | 522 ± 135 | 26 ± 1 | 0.05 |
UTP | NAD+ (10 mm) | 207 ± 26 | 29.5 ± 2 | 0.14 |
NAD+ | ATP (8 mm) | 147 ± 17 | 42 ± 2 | 0.28 |
NADH+ | ATP (8 mm) | n.d. | n.d. | n.d. |
NAAD+ | ATP (8 mm) | n.d. | n.d. | n.d. |