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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Cell Biochem. 2019 Aug 12;121(1):828–839. doi: 10.1002/jcb.29328

Figure 3. Expression of protein markers of apoptotic bodies, microvesicles and exosomes in different EV sub-fractions.

Figure 3

Total protein was isolated from different EV sub-fractions collected from conditioned media of (A) MiaPaCa and (B) AsPC1 cells grown under normoxia or hypoxia (1.0% and 0.1% O2) for 48 h. Following quantification, equal amount of proteins were resolved on SDS-PAGE, blotted on a PVDF membrane and probed by antibodies specific for different marker proteins (THBS1, apoptotic bodies; Arf6, microvesicles; CD9 and CD63, exosomes). (WCL: whole cell lysate, THBS1: thrombospondin)