Total protein was isolated from different EV sub-fractions collected from conditioned media of (A) MiaPaCa and (B) AsPC1 cells grown under normoxia or hypoxia (1.0% and 0.1% O2) for 48 h. Following quantification, equal amount of proteins were resolved on SDS-PAGE, blotted on a PVDF membrane and probed by antibodies specific for different marker proteins (THBS1, apoptotic bodies; Arf6, microvesicles; CD9 and CD63, exosomes). (WCL: whole cell lysate, THBS1: thrombospondin)