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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Gastroenterology. 2019 Aug 20;157(6):1646–1659.e11. doi: 10.1053/j.gastro.2019.08.018

Figure 3. Overexpression of KDM3A induces transformation in non-cancerous pancreatic ductal cells.

Figure 3.

(A) Overexpression of KDM3A in HPNE resulted in forming two transformed clones of Trans21 and Trans18.

(B) Western blot image showing KDM3A overexpression in Trans18 and Trans21 compared to parental HPNE cells and those carrying empty vector (EV).

(C) Increased KDM3A enzyme activities observed in both Trans21 and Trans 18 HPNE clones as well as MiaPaCa2 and S2–007 cells in comparison with HPNE EV (empty vector) cells (p < 0.05)

(D) H3K9Me1 (a substrate of KDM3A) levels are lower and H3K9me2 levels were unchanged among cell lines examined.

(E) Overexpression of KDM3A increased colony formation of Trans 18 and Trans 21 colonies

(F-G) Overexpression of KDM3A increased spheroid forming potential (size and number) of Trans 18 and Trans 21 (p < 0.05).