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. 2019 Sep 17;14(10):2295–2304. doi: 10.1021/acschembio.9b00640

Figure 5.

Figure 5

Chemoproteomic assay for ABHD2 selectivity in mouse testis. (A) Gel-based competitive ABPP with inhibitor 183 (2 and 20 μM) and MAFP (2 μM) against TAMRA-FP (2 μM) in the mouse testis cytosolic and membrane fractions. (* indicates a band reduced in 183 treated vs control.) (B) Competitive chemoproteomic selectivity assay of inhibitor 183 (20 μM) in the testis proteome using ABP FP-biotin (10 μM). (C) Gel-based competitive ABPP with inhibitor 183 (2 and 20 μM) and MAFP (2 μM) against MB064 (2 μM) in the mouse testis cytosolic and membrane fractions. (D) Competitive chemoproteomic selectivity assay of inhibitor 183 (20 μM) in the mouse testis proteome using ABP MB108 (10 μM).