Fig. 3.

Prostate cancer-associated mutants of SPOP are defective in promoting Caprin1 degradation and ubiquitination. a Western blot of indicated proteins in WCLs and samples from co-IP with anti-FLAG antibody in 293 T cells transfected with the indicated plasmids and treated with 20 μM MG132 for 8 h. b Western blot of indicated proteins in WCLs from 293 T cells transfected with the indicated plasmids. c Western blot of the products of in vivo ubiquitination assays from 293 T cells transfected with the indicated plasmids and treated with 20 μM MG132 for 8 h. d Western blot of the indicated proteins in WCLs from C4–2 cells infected with empty vector (EV) or lentivirus expressing wild-type or mutant SPOP. e Western blot of indicated proteins in WCLs and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids and treated with 20 μM MG132 for 8 h. f Western blot of indicated proteins in WCLs from 293 T cells transfected with the indicated plasmids. g Western blot of the products of in vivo ubiquitination assays from 293 T cells transfected with the indicated plasmids and treated with 20 μM MG132 for 8 h