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. 2019 Nov 26;50:98. doi: 10.1186/s13567-019-0715-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of Mycoplasma hyopneumoniae genetic material in laryngeal swabs and tracheobronchial lavage fluid. A Percentage of pigs detected M. hyopneumoniae-positive in laryngeal swabs (LS) and tracheobronchial lavage fluid (TBLF) by real-time PCR. The LS and TBLF were obtained on seven samplings during the 28-day period following experimental inoculation with M. hyopneumoniae. The percentage of pigs detected positive for M. hyopneumoniae in TBLF was significantly higher at 14 and 21 dpi (p < 0.05). B Individual Ct values and mean from PCR detection of M. hyopneumoniae in TBLF samples from 10 inoculated pigs at different days post-inoculation. The Ct values of M. hyopneumoniae-negative samples were presented as 40, which is the arbitrary threshold value of M. hyopneumoniae PCR detection.