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. 2019 Nov 26;50:98. doi: 10.1186/s13567-019-0715-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of Mycoplasma hyopneumoniae infection in serum metabolome. Data from LC–MS analysis of serum samples were processed by principal component analysis (PCA). A Score plot of PCA model. Serum samples collected at 0, 2, 5, 9 dpi were separated from those collected at 14, 21, 28 dpi along the 1st principal component of the model (dashed boxes). Additionally, samples from inoculated pigs at 14 and 21 dpi were further separated from other samples (circled). B Loading plot of PCA model. Major markers contributing to sample separation (I–XX) in the PCA model were identified and labeled in the loading plot and further characterized by structural analysis. The identities of these metabolites are presented in Table 1. C Distribution profile of metabolite markers. The heat map from hierarchical cluster analysis classified the metabolite markers as time-dependent metabolites and infection-responsive metabolites.