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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: ACS Chem Neurosci. 2019 Feb 12;10(7):3197–3206. doi: 10.1021/acschemneuro.8b00638

Figure 1.

Figure 1.

Generation of rs6295 humanized mice. (A) The common G/C single nucleotide polymorphism rs6295 is located upstream of the human HTR1A gene. The transcription factor Deaf1/NUDR binds the C allele but does not bind the G allele. (B) Humanized mice were generated by inserting into the mouse genome a modified bacteria artificial chromosome (BAC) consisting of 180 kb of human DNA containing a duplicated human HTR1A gene containing each allele of rs6295. The rs6295G and rs6295C alleles were isolated by crossing the founder mouse to Cre- and FlpO-recombinase expressing mice (step 1). The offspring of this cross were then bred onto a mouse HTR1A−/− (KO) background (step 2) so that the only source of 5-HT1A in these mice was from human HTR1A locus. Three separate lines were generated.