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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Small. 2019 Jan 28;15(9):e1805182. doi: 10.1002/smll.201805182

Figure 6.

Figure 6.

CCR7 trap downregulated the CCR7 expression in 4T1 tumor cells. a) Immunofluorescence staining and quantification of CCR7 in tumor on day 2 after treatments (green: 4T1 tumor cells, red: CCR7, blue: nucleus; NS: not significant, p ≥ 0.05; ****, p < 0.0001; n = 3). b) Western blot and quantification of CCR7 expression in the tumor. Left panel: on day 2 after one treatment; right panel: on day 0, 2, 4, and 8 after one treatment of CCR7 trap therapy (NS: not significant, p ≥ 0.05; ***, p < 0.001; ****, p < 0.0001; n = 3). c) Western blot and quantification of CCR7, Erk1/2, and AP-1 expression in 4T1 and NMuMG cells after incubation with or without CCR7 trap protein (NS: not significant, p ≥ 0.05; ****, p < 0.0001; n = 3).