Figure 7.

CCR7 trap efficiently inhibited lymphatic metastasis of 4T1 tumor cells prior to tumor resection. a) Photos of tumor resection on 4T1 breast cancer orthotopic model and the removed tumor (upper panel). Bioluminescence imaging before and after tumor resection of 4T1 breast cancer orthotopic model (lower panel). b) Tumor growth curve of the four groups of 4T1 TNBC mice: (1) PBS after resection (mice were treated with PBS on day 9, 11, and 13 (black arrows) after tumor removal surgery on day 7 (green arrow)); (2) Trap after resection (mice were treated with CCR7 trap LPD NPs on day 9, 11, and 13 (black arrows) after tumor removal surgery on day 7 (green arrow)); (3) PBS before resection (mice were treated with PBS on day 9, 11, and 13 (black arrows) before tumor removal surgery on day 15 (green arrow)); (4) Trap before resection (mice were treated with CCR7 trap LPD NPs on day 9, 11, and 13 (black arrows) before tumor removal surgery on day 15 (green arrow)) (NS: not significant, p ≥ 0.05; ****, p < 0.0001; n = 6). c) Bioluminescence imaging of sentinel LNs and contralateral LNs at the endpoint. d) Immunofluorescence staining of 4T1 tumor cell metastasis in sentinel LNs at the endpoint (green: 4T1 tumor cells, red: LYVE1, blue: nucleus). e) Flow cytometry measuring green fluorescence signals of 4T1 cells in sentinel LNs at the endpoint (*, p < 0.05; n = 6).