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. 2019 Nov 26;38:474. doi: 10.1186/s13046-019-1442-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — IL-6 activates the expression of DNMTs and OCT4 in vitro and in vivo. a The effect of IL-6 (50 ng/mL) on mRNA expression of DNMTs and OCT4 in HBV⁺HBsAg⁺ HCCs (Hep3B and HepG2.2.15) and HBV⁻HBsAg⁻ HCCs (HepG2) under different incubation time assessed by real-time qPCR. b The effect of IL-6 (50 ng/mL) on the protein expression of DNMTs and OCT4 in HBV⁺HBsAg⁺ HCCs (Hep3B and HepG2.2.15) and HBV⁻HBsAg⁻ HCCs (HepG2 and Huh7) by western blotting. The qualitative data is shown in (c). Three individual experiments were carried out for each experimental condition. *P < 0.05, **P < 0.01, ***P < 0.0001, student t test. d Xenografts of 5 × 10⁶ Hep3B cells in athymic nude mice with IL-6 (200 ng/mouse/3 d) treatment (n = 6). Immuno-histochemical staining for p-STAT3, DNMT3b, OCT4, DNMT1, DNMT3a, and the control IgG are shown (Bar, 100 μm)