Table 1.

Variables associated with early tumor recurrence after hepatectomy for HCC (N = 144)

Variable	Recurrencea
	Univariate analysis P value	Multivariate analysis
		P value	Hazard ratio	95% confidence interval
Gender (male vs. female)	0.154	–	–	–
Age, years (<  55 vs. ≥ 55)	0.707	–	–	–
HBV (yes vs. no)	0.540	–	–	–
HCV (yes vs. no)	0.647	–	–	–
Bilirubin, mg/dL (<  1.2 vs. ≥ 1.2)	0.863	–	–	–
Albumin, g/dL (< 3.5 vs. ≥ 3.5)	0.252	–	–	–
ALT, U/L (<  35 vs. ≥ 35)	0.207	–	–	–
PT, INR (<  1.2 vs. ≥ 1.2)	0.075	0.104	2.827	0.808–9.885
ICG, retention rate (<  15% vs. ≥ 15%)	0.170	–	–	–
TNM stage (T1/2 vs. T3/4)	0.417	–	–	–
Multiple tumors (yes vs. no)	0.739	–	–	–
Child-Pugh class (A vs. B)	0.251	–	–	–
Complete tumor capsule (yes vs. no)	0.051	0.081	0.418	0.157–1.112
Microvascular invasion (yes vs. no)	0.001b	0.398	1.468	0.603–3.578
Macrovascular invasion (yes vs. no)	< 0.001b	0.002b	11.993	2.553–56.339
Cut margin free (yes vs. no)	0.873	–	–	–
Differentiation grade (1/2 vs. 3/4)	0.168	–	–	–
Tumor size, cm (<  3 vs. ≥ 3)	0.039b	0.554	0.788	0.359–1.733
Satellite nodules (yes vs. no)	0.008b	0.969	1.019	0.393–2.640
AFP, ng/mL (<  400 vs. ≥400)	0.625	–	–	–
Serum IL-6, ng/mL (<  150 vs. ≥ 150)	0.023b	0.088	1.896	0.910–3.949
Serum TGF-β (<  14,000 vs. ≥ 14,000)	0.682	–	–	–
DNMT1 (< 2X vs. ≥ 2X)	0.010b	0.719	1.144	0.550–2.378
DNMT3a (< 2X vs. ≥ 2X)	0.305	0.239	1.632	0.722–3.689
DNMT3b (< 2X vs. ≥ 2X)	0.037b	0.199	1.779	0.739–4.285
OCT4 (< 2X vs. ≥ 2X)	0.026b	0.085	1.990	0.909–4.357
NANOG (< 2X vs. ≥ 2X)	0.004b	0.010b	3.074	1.309–7.220
High expression of OCT4 and DNMT3b (< 2X vs. ≥ 2X)	0.001b	0.395	1.484	0.597–3.685
High expression of OCT4 and DNMT1 (< 2X vs. ≥ 2X)	0.013b	0.226	1.754	0.705–4.364

Abbreviations: AFP serum α-fetoprotein, ALT alanine aminotransferase, ICG indocyanine green, PT prothrombin time, INR international normalized ratio, Tumor size, the largest one if multiple

^aTime to early recurrence (less than 2 years)

^bP < 0.05.