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. 2019 Nov 26;11:162. doi: 10.1186/s13148-019-0751-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Increasing maternal age plus ARTs did not further increase imprinted methylation errors at the (a) Snrpn, (b) Kcnq1ot1, and (c) H19 ICRs in blastocysts. Diamonds represent the mean methylation levels of maternal (red) or paternal (blue) alleles for Snrpn, Kcnq1ot1, and H19 ICRs in individual embryos (n = 10–17 blastocysts; n = 2–5 females per treatment group). Letters (a, b, c), a statistically significant difference between groups (Additional file 1: Table S2)