Fig. 2.

AK002-G4 selectively binds to eosinophils and basophils in human peripheral blood. a Flow cytometry gating strategy used to identify immune cell subtypes among human PBL. b AK002-G4 binding plotted as ΔMFI on immune cells identified using gating strategy in a (mean ± SD of 4 donors). c Representative histograms for eosinophils, basophils, neutrophils, and monocytes stained with an FMO control (gray) or AK002-G4 (blue). d Siglec-8 ABC of peripheral blood eosinophils (median 18,029 ± 6,133) and basophils (median 552 ± 292) determined by quantitative cytometry (individual donors are plotted ± SD). ΔMFI was determined by subtracting the MFI for a FMO control sample from the MFI for cells stained with the conjugated antibody. ΔMFI values that were negative after subtracting the FMO were given a value of zero. ABC, antibody binding capacity; MFI, median fluorescence intensity; FMO, fluorescence minus one; NK, natural killer.