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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: Cell Rep. 2019 Sep 17;28(12):3047–3060.e7. doi: 10.1016/j.celrep.2019.08.037

Figure 2. Co-inhibitory Receptor Expression and the Functional Potential of CD4+ T Cell Subsets in HIV-Infected LNs.

Figure 2.

(A) Relative expression of the indicated co-inhibitory receptors as a percentage of indicated subsets (n = 8).

(B) Representative staining for IFN-γ and TNF-α in PD-1− and PD-1+/++ T cells. PD-1− cells combined CXCR5−PD-1− and CXCR5+PD-1− subsets. PD-1+/++ cells included both CXCR5− and CXCR5+ cells of intermediate (PD-1+) and high PD-1 expression (PD-1++).

(C) The frequency of cells in individual subsets that positively stained for the indicated cytokines after PMA and ionomycin stimulation (n = 8).

(D) The frequency of IL-21+ T cells in the indicated subsets following stimulation as measured by CyTOF (n = 25).

For (A), Friedman test was applied and corrected using Dunn’s multiple comparison test. For (C), two-way ANOVA was performed. Mean of each subset were compared to that of CXCR5−PD-1− T cells and corrected using Dunnett’s multiple comparisons test. For (D), one-way ANOVA was performed and corrected using Holm-Sidak method. Data are represented as mean ± SEM.

Also see Figures S3 and S4A–S4C.