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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: Cell Rep. 2018 Oct 30;25(5):1225–1240.e6. doi: 10.1016/j.celrep.2018.10.012

Figure 1. HITS-CLIP Identification of DAZL-RNA Contacts in the Adult Testis.

Figure 1.

(A) Autoradiograph of radiolabeled cross-linked DAZL-RNA complexes purified from adult testes. Arrowhead indicates DAZL cross-linked to minimal RNA fragments. Bracket indicates RNA fragments of 35–50 nt excised for cDNA preparation.

(B) Venn diagram of overlapping CLIP reads from each replicate and identification of 11,297 genomic coordinates with overlapping CLIP reads from 3/3 biological replicates.

(C) Bins of CLIP peaks normalized to RNA-seq, with GUU enrichment (green) and proportion of peaks in each bin with GUU (gray), and P values from χ2 distribution of GUU enrichment at right.

(D) Top motifs present in bin 1, identified by MEME (top, middle) and DREME (bottom).

(E) Top motifs associated with CIMS sites identified by MEME.

(F) Top 10 6mers and 4mers associated with CIMS sites.

(G) Positioning of UGUUGU, GUUG, and UGUU motifs relative to CIMS deletion sites.