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. 2019 Nov 19;13:1225. doi: 10.3389/fnins.2019.01225

FIGURE 7.

FIGURE 7

Repetitive transcranial magnetic stimulation (rTMS) does not affect the stemness of bone mesenchymal stromal cells (BMSCs). (A) Cell viability was assessed by Cell Counting Kit-8 assays. n = 3–5. All data were analyzed with a one-way ANOVA followed by Dunnett’s multiple comparison test. NS, not significant. (B) Nanog, Oct4, and Sox2 were assessed by western blotting. (C–E) Quantification of western blotting for Nanog, Oct4, and Sox2. Data were analyzed with a one-way ANOVA followed by Dunnett’s multiple comparison test. NS, not significant. n = 3. Error bar = SD. (F) phosphorylated epidermal growth factor receptor (p-EGFR), EGFR, p-PKA, and PKA were assessed by western blotting. (G,H) Quantification of western blotting for p-EGFR/EGFR and p-PKA/PKA. Data were analyzed with a one-way ANOVA followed by Dunnett’s multiple comparison test. NS, not significant. n = 3. Error bar = SD. (I) Schematic diagram of the autophagy pathway induced by rTMS in BMSCs.