Fig. 3.

CORT108297 and CORT118335 induce GR binding to coregulators in an intermediate fashion compared to dexamethasone and RU486. Each column represents a unique coregulator-derived peptide from a range of 50 nuclear receptor coregulators. In the MARCoNI analysis, the peptides were immobilized on a solid support and incubated with cell lysates containing tagged-GR, a ligand (dexamethasone, CORT108297, CORT118335, or RU486), and a tag-specific antibody coupled with a fluorophore. The relative interaction between each peptide and the GR was assessed by detecting the fluorescent label. The modulation index represents the log₁₀-transformed ratio of the normalized fluorescence value. The values were normalized to the values obtained in control conditions (with dimethyl sulfoxide treatment) [unpubl. data].