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. 2019 Nov 4;18(6):4617–4624. doi: 10.3892/etm.2019.8166

Figure 3.

Figure 3.

Effect of miR-195-5p downregulation on HUVECs. (A) After transfection with inhibitor control or miR-195-5p inhibitor for 48 h, the level of miR-195-5p in HUVECs was detected using RT-qPCR. (B) After transfection with inhibitor control or miR-195-5p inhibitor for 48 h, the cell viability of HUVECs was detected using MTT assay. (C) After transfection with inhibitor control or miR-195-5p inhibitor for 48 h, cell apoptosis of HUVECs was detected using flow cytometry, and the cell apoptosis rate (Q2 + Q4) was calculated and presented. After transfection with inhibitor control or miR-195-5p inhibitor for 48 h, the mRNA level of (D) Bcl-2 and (E) Bax in HUVECs was detected using RT-qPCR. (F) After transfection with inhibitor control or miR-195-5p inhibitor for 48 h, the protein level of Bcl-2 and Bax in HUVECs was detected using western blotting. Control, HUVECs without any treatment; inhibitor control, HUVECs transfected with inhibitor control for 48 h; inhibitor, HUVECs were transfected with miR-195-5p inhibitor for 48 h. Data are presented as the mean ± SD. *P<0.05, **P<0.01 vs. inhibitor control. HUVECs, human umbilical vein endothelial cells; miR, microRNA; PI, propidium iodide; Q, quadrant; RT-qPCR, reverse transcription-quantitative PCR.