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. 2019 Nov 4;18(6):4617–4624. doi: 10.3892/etm.2019.8166

Figure 4.

Figure 4.

Effect of miR-195-5p upregulation on HUVECs. (A) After transfection with mimic control or miR-195-5p mimic for 48 h, the level of miR-195-5p in HUVECs was detected using RT-qPCR. After transfection with control-plasmid or Bcl-2-plasmid for 48 h, the mRNA and protein level of Bcl-2 in HUVECs was detected using (B) RT-qPCR and (C) western blotting. (D) After transfection with mimic control, miR-195-5p mimic or miR-195-5p mimic + Bcl-2-plasmid for 48 h, the cell viability of HUVECs was detected using MTT assay. (E) After transfection with mimic control, miR-195-5p mimic or miR-195-5p mimic + Bcl-2-plasmid for 48 h, the cell apoptosis of HUVECs was detected using flow cytometry, and the cell apoptosis rate (Q2 + Q4) was calculated and presented. (F) After transfection with mimic control, miR-195-5p mimic or miR-195-5p mimic + Bcl-2-plasmid for 48 h, the protein level of Bcl-2 and Bax in HUVECs was detected using western blotting. After transfection with mimic control, miR-195-5p mimic or miR-195-5p mimic+Bcl-2-plasmid for 48 h, the mRNA level of (G) Bcl-2 and (H) Bax in HUVECs was detected using RT-qPCR. Control, HUVECs without any treatment; mimic control, HUVECs transfected with mimic control for 48 h; miR-195-5p mimic, HUVECs transfected with miR-195-5p mimic for 48 h; control-plasmid, HUVECs transfected with control-plasmid for 48 h; Bcl-2-plasmid, HUVECs transfected with Bcl-2-plasmid for 48 h; miR-195-5p mimic+Bcl-2-plasmid, HUVECs co-transfected with miR-195-5p mimic + Bcl-2-plasmid for 48 h. Data are presented as the mean ± SD. **P<0.01 vs. mimic control or control-plasmid; ##P<0.01 vs. miR-195-5p mimic. HUVECs, human umbilical vein endothelial cells; miR, microRNA; PI, propidium iodide; Q, quadrant; RT-qPCR, reverse transcription-quantitative PCR.