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. 2019 Nov 19;10:2674. doi: 10.3389/fimmu.2019.02674

Figure 1.

Figure 1

Macrophages but not monocytes are highly IFN-λ3 responsive. To investigate IFN-λ responsiveness, immune cell subsets were magnetically isolated and IFNLR1 expression was quantified by ddPCR (A). Mϕ and pDC IFNLR1 expression was significantly higher than monocyte, NK and T cell populations (p < 0.05, n = 8). Time course analysis demonstrated that IFNLR1 expression quickly rises following monocyte plating, reaching a 30× increase at 24 h even in the absence of GM-CSF addition (p < 0.001, n ≥ 5) (B). Similarly, IFNLR1 surface expression during macrophage differentiation (MFI) increased at days 3 and 7 (p < 0.001, n ≥ 5) (C). Isolated immune cell subsets were treated with 100 ng/ml IFN-λ3 for 8 h and the expression of ISGs viperin and ISG15 were examined (n ≥ 7) (D). Consistent with IFNLR1 expression, Mϕs and pDCs were highly responsive to IFN-λ3, whereas monocytes and NK cells were not (n ≥ 5). Data are representative of two (B,C) and three independent experiments (A,D). One-way ANOVA (A), Mann–Whitney test (B,D), paired t-test (C), *p < 0.05, **p < 0.01, ***p < 0.001 (mean ± SE).