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. 2019 Nov 19;10:2674. doi: 10.3389/fimmu.2019.02674

Figure 2.

Figure 2

M-CSF and GM-CSF differentiated macrophages respond differently to IFN-α and IFN-λ3. Following M- and GM-CSF stimulated differentiation, IFN-λ responsiveness of Mϕ populations was examined, and compared to IFN-α. M-CSF and GM-CSF Mϕ subsets both increased the expression of IFNAR1 ~2-fold following differentiation from monocytes, and IFNLR1 transcripts ~30- and 60-fold, respectively (A) (n ≥ 9). Western blot of IFNAR1 and IFNLR1 from four healthy subjects confirmed these findings (B) (n = 7, total). Supporting these findings, phosphorylation of STAT1 was detected by Western blot in both monocytes and macrophages following 15 min of IFN-α treatment and macrophages only following IFN-λ3 (C). ISG transcripts for ISG15, viperin, CD80, and TRAIL were examined to measure Mϕ sensitivity to IFN-α (50 U/ml) and IFN-λ3 (100 ng/ml) (D) (n = 8). M-CSF differentiated Mϕs were more responsive to IFN-α, whereas GM-CSF differentiated Mϕs, IFN-λ3. Data are representative of two independent experiments. Paired t-test */#p < 0.05, **/##p < 0.01, ***p < 0.001 (mean ± SE). *Mock vs. IFN treatments, #M-Mϕ vs. GM-Mϕ.