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Validation of differentially expressed genes (DEGs) using quantitative real-time reverse transcription PCR (qRT-PCR). The qRT-PCR measurements of the expression of RFC3, ADRA2A, WWC1, SPP1, and FCGBP mRNA transcripts were analyzed using the ΔΔCt method; *significant difference between the high-RFI and low-RFI groups (P < 0.05), **very significant difference between the high-RFI and low-RFI groups (P < 0.01).
