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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Sep 13;1865(12):165557. doi: 10.1016/j.bbadis.2019.165557

Figure 7: In vitro cholangiocyte SCF secretion and MC migration.

Figure 7:

By EIA we found that cholangiocyte SCF secretion decreases after inhibition using ISCK03 (10 μM, 24 hrs) (A). Boyden chambers were loaded with cholangiocytes treated either with 0.1% BSA (control) or ISCK03 (10 μM, 24 hrs) into the bottom chamber and cultured MCs were placed in the upper chamber and MC migration was measured after 24 hrs (B). MC migration was significantly blunted when cholangiocytes were treated with the SCF inhibitor, ISCK03 compared to control as shown by Toluidine Blue staining (C). Data are mean ± SEM of 9–12 experiments for EIA and 3 experiments for migration. *p<0.05 vs. cholangiocyte control. Representative images x20.