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. Author manuscript; available in PMC: 2021 Jan 16.
Published in final edited form as: J Proteomics. 2019 Nov 1;211:103544. doi: 10.1016/j.jprot.2019.103544

Figure 1. Total HDX of intact proteins in free and ligand-bound states.

Figure 1.

A. Spy L32P +/− Im7–45 analysis. B. FKBP25 F145A/I223P +/− rapamycin analysis. The number of protected protons, which reflects the total content of secondary structure, is very low in the ligand-free mutant proteins and dramatically increases upon ligand binding.