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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: Cell Rep. 2019 Sep 24;28(13):3497–3509.e4. doi: 10.1016/j.celrep.2019.08.051

Figure 3. Loss-of-Function Screens Identify Proteins Important for the Response to Replication Stress.

Figure 3.

(A) Schematic for performing loss-of-function screens. Genes were considered hits if 2 or more of the 4 siRNAs yielded the same effect.

(B) Number of gene knockdowns resulting in viability less than 5% of control.

(C) Numbers of gene knockdowns resulting in resistance or sensitivity to ATRi, CPT, and HU.

(D) Heatmaps of the average viability of cells transfected with each siRNA as compared to a control siRNA in the absence of added replication stress. Each row is a single gene and each column is one of four siRNAs.

(E) Average viability of cells transfected with each siRNA and treated with the indicated drugs as compared to a control siRNA.

See also Figure S3.