Skip to main content
. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: Cell Rep. 2019 Sep 24;28(13):3497–3509.e4. doi: 10.1016/j.celrep.2019.08.051

Figure 5. BRD2, 3, and 4 Are Replication Fork Proteins Required for Fork Progression.

Figure 5.

(A) U2OS cells were transfected with non-targeting (siNT) or BRD3 siRNAs, treated with increasing concentrations of ATR inhibitor (VX-970) and measured for viability after 72 h. Mean ± SD, n = 3, two-way ANOVA with Dunnett post-test.

(B) Immunoblot analysis to verify siRNA knockdown.

(C–F) Fork speeds were measured in (C, E, and F) U2OS or (D) RPE-hTERT cells transfected with siRNAs and labeled with IdU and CldU as indicated. Mean, ANOVA with Dunnett post-test (C, D, and F) or two-tailed t test (E). In (F) cells were pre-incubated with DRB for 1 h prior to labeling and maintained during labeling. siRNA pools were used in (D) and (E).

See also Figure S4.