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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: Cell Rep. 2019 Sep 24;28(13):3497–3509.e4. doi: 10.1016/j.celrep.2019.08.051

Figure 6. BET Proteins Interact with the ATAD5/RFC2–5 Complex.

Figure 6.

(A) FLAG-HA-BRD3 was immunopurified from 293T cells and interacting proteins identified by mass spectrometry. The table indicates the number of peptides identified for each protein. The control is 293T cells that do not express any tagged protein.

(B and C) FLAG-HA-BRD3 (B) or FLAG-ATAD5 (C) was immunopurified from 293T cells and immunoblotted as indicated.

(D) Schematic of BET family proteins and fragments utilized. The ability of each GFP-HA-NLS-tagged protein to interact with ATAD5 as measured in (C) and (E) is indicated.

(E and F) GFP-HA-NLS-tagged fragments (E) or constructs (F) were immunopurified from 293T cells and immunoblotted as indicated.

See also Figure S4.