KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti PCNA | Santa Cruz Biotechnology | Cat#sc-56; RRID:AB_628110 |
| Mouse monoclonal anti FLAG M2 | Sigma-Aldrich | Cat#F3165; RRID:AB_259529 |
| Mouse monoclonal anti BRD3 | Abeam | Cat#ab50818; RRID:AB_868478 |
| Mouse monoclonal anti GAPDH | Millipore | Cat#MAB374; RRID:AB_2107445 |
| Rabbit anti ATAD5 | Gift from Kyungjae Myung | Lee et al., 2010 |
| Rabbit polyclonal anti RFC1 | Abeam | Cat#ab3853; RRID:AB_2238314 |
| Rabbit monoclonal anti RFC4 | Abeam | Cat#ab192021 |
| Rabbit polyclonal anti H2B | Abeam | Cat#ab1790; RRID:AB_302612 |
| Rabbit monoclonal anti BRD2 | Abeam | Cat#ab139690; RRID:AB_2737409 |
| Rabbit polyclonal anti BRD4 | Bethyl | Cat#A301–985A50; RRID:AB_2631449 |
| Rabbit polyclonal anti GFP | Santa Cruz | Cat#se-8334; RRID:AB_641123 |
| Rat monoclonal anti BrdU | Abeam | Cat#ab6326; RRID:AB_305426 |
| Mouse monoclonal anti BrdU | BD Bioscienees | Cat#347580; RRID:AB_400326 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| EdU | VICB Synthesis Core | N/A |
| VX-970 | Selleck | Cat#S7102 |
| Camptothecin | Sigma Aldrich | Cat#C9911 |
| 13C615N2 L-Arginine | Cambridge Isotope Laboratories | CNLM-539-H |
| 13C615N4 L-Lysine | Cambridge Isotope Laboratories | CNLM-291-H |
| JQ1 | Selleck | Cat#S7110 |
| GFP-Trap Magnetic Agarose | Chromotek | Cat#gtma-10 |
| IdU | Sigma | Cat#l7125 |
| CldU | Sigma | Cat#C6891 |
| Experimental Models: Cell Lines | ||
| U2OS | ATCC | Cat#HTB-96; RRID:CVCL_0042 |
| HEK293T | ATCC | Cat#CRL-11268; RRID:CVCL_1926 |
| hTERT-RPE | ATCC | Cat#CRL-4000; RRID:CVCL_4388 |
| HCT-116 | ATCC | Cat#CCL-247; RRID:CVCL_0291 |
| HeLa | ATCC | Cat#CCL-2; RRID:CVCL_0030 |
| Kasumi-1 | ATCC | Cat#CRL-2724; RRID:CVCL_0589 |
| Oligonucleotides | ||
| Screen siRNAs | Dharmacon | Refer to Table S7 |
| BRD3 siRNA 2 | Dharmacon | D-004936–02 |
| BRD3 siRNA 17 | Dharmacon | D-004936–17 |
| BRD2 siRNA pool | Dharmacon | M-004935–02 |
| BRD4 siRNA pool | Dharmacon | M-004937–02 |
| ATAD5 siRNA pool | Dharmacon | M-004738 |
| BRD2 siRNA 8 | Dharmacon | J-004935–08 |
| BRD2 siRNA 9 | Dharmacon | J-004935–09 |
| BRD4 siRNA 8 | Dharmacon | J-004937–08 |
| BRD4 siRNA 9 | Dharmacon | J-004937–09 |
| Recombinant DNA | ||
| BRD3 plasmid | This paper | N/A |
| BRD2 plasmid | Addgene | Addgene Cat#65376; Gong et al., 2015 |
| BRD4 plasmid | Addgene | Addgene Cat#14441; You et al., 2004. |
| Software and Algorithms | ||
| Graphpad Prism | Graphpad Software |
https://www.graphpad.com/scientific-software/prism/
RRID: SCR_000306 |
| Perseus | Perseus | Tyanova et al., 2016; RRID:SCR_015753 |