Table 1:
Primer sequences.
| Primer* | Sequence | Notes |
|---|---|---|
| SPP-N-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Kozak sequence present |
| SPP-Asp-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Same as SPP-N-F |
| SPP-Asp-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATCATATGTGTCTACTGTGGGGACA | Without RGD sequence |
| SPP-SVV-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Same as SPP-N-F |
| SPP-SVV-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCCAGTCCATAAACCACACTATCACCT | |
| SPP-Thr6-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Same as SPP-N-F |
| SPP-Thr6-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCTTTTGACCTCAGTCCATAAACCACAC | |
| SPP-Tail7-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Same as SPP-N-F |
| SPP-Tail7-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCAGAAGGCGCGTTCAGGTCCT | |
| SPP-Ca+8-F | GGGATCCGCCACAACCATGAGAATTGCAGTGATTTGCTTTTGCCT | Same as SPP-N-F |
| SPP-Ca+8-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCCGTTTCATAACTGTCCTTCCCACG | |
| SPP-Nr2-F | CACCGGAGCTCGCCACAACCATGGAACGACTCTGATGATGTAGATGACAC | |
| SPP-Nr2-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | |
| SPP-Aspr3-F | CACCGGAGCTCGCCACAACCATGGACTCTGATGATGTAGATGACACTGAT | |
| SPP-Aspr3-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | Same as SPP-Nr2-R |
| SPP-RGD-F | CACCGGAGCTCGCCACAACCATGGGCCGAGGTGATAGTGTGGTTTAT | |
| SPP-RGD-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | Same as SPP-Nr2-R |
| SPP-SVVr-F | CACCGGAGCTCGCCACAACCATGAGTGTGGTTTATGGACTGAGGTCAAAA | |
| SPP-SVVr-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | Same as SPP-Nr2-R |
| SPP-ThrΔ-F | GGAGCTCGCCACAACCATGAAATCTAAGAAGTTTCGCAGACCTGACA | |
| SPP-ThrΔ-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | Same as SPP-Nr2-R |
| SPP-Ca2-F | GGAGCTCGCCACAACCATGGATTGGGACAGCCGTGGGAA | |
| SPP-Ca2-R | GCCTCGAGTTATTTATCGTCGTCATCCTTATAATCATTGACCTCAGAAGA | Same as SPP-Nr2-R |
*
Bam HI restriction site at N-termini and Xho I at C-terminus with FLAG epitope and stop codon.