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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Physiol Behav. 2019 Oct 16;212:112705. doi: 10.1016/j.physbeh.2019.112705

Figure 1.

Figure 1.

Experimental design and induction of status epilepticus (SE). (A) Timeline of the experimental design. Following pilocarpine-induced SE induction (day 0) and C1 esterase inhibitor (C1-INH) treatment (20 Units/kg (i.p.) on days 0, 1, and 2) rats were subjected to a battery of behavioral tests (days 3-22) including Open Field (OF), Novel Object Recognition (NOR), Barnes Maze (BM) and Probe trial (PT). Brain samples were collected at 3 and 14 days after SE for immunohistochemistry (IHC), Western blotting (WB), or solid-based immunoassay (SBI). Rats’ weights were recorded daily from day 0-14 and on day. Behavioral seizures were monitored for up to two hours after pilocarpine injection. All rats included in the study reached SE score of at least 4.5.