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Southern blot restriction digests and probe sequences
| gRNA target site | Restriction digestion enzyme(s) | Oligonucleotide sequence (5′–3′) |
|
|---|---|---|---|
| Forward probe | Reverse probe | ||
| Eb | HindIII | CTAGTCCCATCACCTAAGTCCCAG | GTCAGCAAATGTTCATAGAGC |
| Irf4 intron | KpnI | GGCCTCAGTCTGTGTGGGG | GAGAGCCCAGCTAGCATGGG |
| Irf4 5′ | BamHI | GCATTGTCCTACCTCCAAAGAC | CTAGGTTCCTTATGACCATGG |
| Ifit1 intron | EcoRI/BamHI | GCCATTCATGAATTTCAAGAGAC | GAGCCTCAGTGCCTTCTCATAG |
| Ifit1 3′ | KpnI/EcoRI | GCCATTCATGAATTTCAAGAGAC | GAGCCTCAGTGCCTTCTCATAG |
