TABLE 3.
RT-qPCR and ChIP-qPCR primer sequences
| Primer name | Oligonucleotide sequence (5′–3′) |
|
|---|---|---|
| Forward | Reverse | |
| β-2-Microglobulin | GGTCTTTCTGGTGCTTGTCTCA | GTTCGGCTTCCCATTCTCC |
| Irf4 P1 | AAGCAGGACTACAATCGTGAG | TCGGAACTTGCCTTTAAACAATG |
| Irf4 P2 | CAGAGACAGAGGAAGCTCATC | GTGTTCAGGTAACTCGTAGCC |
| Ifit1 P1 | CAAGAGAGCAGAGAGTCAAGG | GTGGTAGCTGTTCTCTGGG |
| Ifit1 P2 | AGAGTCAAGGCAGGTTTCTG | TGTGAAGTGACATCTCAGCTG |
| Ifit1 P3 | TCTAGCAGGCAATTCCATCC | TCCAAAGGCACAGACATAAGG |
| Ifit3 | CCTCTGCTCATCCTAGCCATACA | GTGCTCTGTCTGCTTTAAGCTC |
| Ifit3b | GAGGTTAAACAGCAAACATAGG | CCCTAACCCATTCAGCCACTC |
| Ifit2 | CATTAGAAGGCAGAGGAAGAGG | GGCATTTTAGCTGTCGCAG |
| Kif20b | AGGAAGTGGAATTCTGCAGG | AAAACGTAAGACGGTCTAGGC |
| Stambpl1 | CCGAGATGAAGTGACTGAGAAG | CTGTATGGTCAGGCATAGCAG |
| Atad | GACCACGAAGCTACAGCGAT | CTTGAGGGCGATTGGTAG |
| Tnks2 | ACCCAAATGCTCGGGATAAT | GGCTCAGCTCCATGCTGTAA |
| Isg15 | GCAGACTCCTTAATTCCAGGG | TTCAGTTCTGACACCGTCATG |
| Ifit1 ChIP P1 | GGCATCTTCCTTCCATTACTTG | GACCACAGAAAGAGCATCAGTG |
| Isg15 ChIP | CCACAGCAACATCTATGAGGTC | ACTGGTCTTCGTGGACTTGTTC |