FIG 3.

Stressed RNA granule pellet transcriptomes are reproducible. (A) 3D scatterplot depicting the normalized read counts from RNA-seq libraries from stressed RNA granule pellet triplicates. (B) 3D scatterplot depicting the normalized read counts from RNA-seq libraries from stressed total RNA triplicates. (C) Table depicting pairwise Pearson correlation coefficients between RNA granule pellets and total RNA in stressed cells. (D) MA plot depicting the log₂ fold change values (stressed RG pellet/stressed total RNA) versus abundance (FPKM). Genes are color-coded by their significance. Significant genes (P < 0.01) are colored red, while nonsignificant (P > 0.01) genes are colored blue. (E) Scatterplot of stressed RG enrichment obtained from mouse fibroblasts versus stressed RG enrichment in U-2 OS cells. (F) Gene ontology for enriched and depleted transcripts.