Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Danio rerio) | Tübingen | ZIRC | RRID: ZIRC_ZL57; ZFIN ID: ZDB-GENO-990623–3 | |
| Strain, strain background (Danio rerio) | TL | ZIRC | RRID: ZIRC_ZL86; ZFIN ID: ZDB-GENO-990623–2 | |
| Genetic reagent (Danio rerio) |
Tg(myo6b:GCaMP6s-CAAX)idc1;
GCaMP6sCAAX; 6sCAAX; GCaMP6s |
(Jiang et al., 2017) | RRID: ZFIN_ZDB-ALT-170113-3; https://zfin.org/ZDB-ALT-170113-3 | Membrane-localized calcium biosensor |
| Genetic reagent (Danio rerio) | Tg(myo6b:RGECO1)vo10Tg; CytoRGECO1 | (Maeda et al., 2014) | RRID: ZFIN_ZDB-ALT-150114-2; https://zfin.org/ZDB-ALT-150114-2 | Cell-fill calcium biosensor |
| Genetic reagent (Danio rerio) | Tg(myo6b:GCaMP3)w78Tg; CytoGCaMP3 | (Esterberg et al., 2013) | RRID: ZFIN_ZDB-ALT-130514-1; https://zfin.org/ZDB-ALT-130514-1 | Cell-fill calcium biosensor |
| Genetic reagent (Danio rerio) | Tg(myo6b:mitoGCaMP3)w119Tg; MitoGCaMP3; Mito | (Esterberg et al., 2014) | RRID: ZFIN_ZDB- ALT-141008–1; https://zfin.org/ZDB-ALT-141008-1 | Mitochondria-localized calcium biosensor |
| Genetic reagent (Danio rerio) | Tg(myo6b:ribeye a-tagRFP)idc11Tg; Rib a-RFP; Rib a-tagRFP; Rib a | (Sheets, 2017) | RRID: ZFIN_ZDB- ALT-190102–4; https://zfin.org/ZDB-ALT-190102-4 | Ribbon-localized fluorescent protein |
| Genetic reagent (Danio rerio) | Tg(myo6b:mitoRGECO1)idc12Tg; MitoRGECO1 | This paper | RRID: ZFIN_ZDB-ALT-190102-5; https://zfin.org/ZDB-ALT-190102-5 | Mitochondria-localized calcium biosensor. See Materials and methods,‘Cloning and Transgenic Fish Production’ |
| Genetic reagent (Danio rerio) | Tg(myo6b:Rex-YFP)idc13Tg; Rex-YFP | This paper | RRID: ZFIN_ZDB- ALT-190102–6; https://zfin.org/ZDB-ALT-190102-6 | Cell-fill NAD+/NADH ratio biosensor. See Materials and methods, ‘Cloning and Transgenic Fish Production’ |
| Antibody | Ribbon label: Mouse anti-Ribeye b IgG2a; Ribeye b; Ribeye; Rib b |
(Sheets et al., 2011) | N/A | 1:10,000 |
| Antibody | PSD label: Mouse anti-pan-MAGUK IgG1; MAGUK | NeuroMab | RRID: AB_10673115; K28/86, #75–029 | 1:500 |
| Antibody | Hair cell label: Rabbit anti-Myosin VIIa | Proteus | RRID: AB_10015251; #25–6790 | 1:1000 |
| Antibody | goat anti-mouse IgG2a, Alexa Fluor 488 | ThermoFisher Scientific | RRID: AB_2535771; #A-21131 | 1:1000 |
| Antibody | goat anti-rabbit IgG (H+L) Alexa Fluor 568 | ThermoFisher Scientific | RRID: AB_143157; #A-11011 | 1:1000 |
| Antibody | goat anti-mouse IgG1 Alexa Fluor 647 | ThermoFisher Scientific | RRID: AB_2535809; #A-21240 | 1:1000 |
| Recombinant DNA reagent | Plasmid: 5E-pmyo6b | (Kindt et al., 2012) | N/A | |
| Recombinant DNA reagent | Plasmid: 3E-polyA | (Kwan et al., 2007) | #302 | |
| Recombinant DNA reagent | Plasmid: pDestTol2CG2 | (Kwan et al., 2007) | #395 | |
| Recombinant DNA reagent | Plasmid: pC1-Rex-YFP | (Bilan et al., 2014) | Addgene #48247 | |
| Recombinant DNA reagent | Plasmid: pDONR221 | ThermoFischer | Cat #12536017 | |
| Recombinant DNA reagent | Plasmid: CMV-R-GECO1 | (Zhao et al., 2011) | Addgene #32444 | |
| Recombinant DNA reagent | Plasmid: pME-Rex-YFP | This paper | N/A | See Materials and methods, ‘Cloning and Transgenic Fish Production’ |
| Recombinant DNA reagent | Plasmid: pME-mitoRGECO1 | This paper | N/A | See Materials and methods, ‘Cloning and Transgenic Fish Production’ |
| Sequence-based reagent | RexYFP attB FWD | This paper | PCR primers | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCGCCACCATGAAGGTCCCCGAAGCG; Made by Integrated DNA Technologies (IDT). |
| Sequence-based reagent | REX-YFP attB REV | This paper | PCR primers | GGGGACCACTTTGTACAAGAAAGCTGGGTGTCACCCCATCATCTCTTCCCG |
| Sequence-based reagent | RGECO1 FWD1 | This paper | PCR primers | [ATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCA]-GTCGACTCTTCACGTCGTAAGTG; Made by IDT. |
| Sequence-based reagent | RGECO1 REV1 | This paper | PCR primers | CTACTTCGCTGTCATCATTTGTACAAACTC; Made by IDT. |
| Sequence-based reagent | RGECO1 attB FWD2 | This paper | PCR primers | GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCCGTCCTGACGCCGC; Made by IDT. |
| Sequence-based reagent | RGECO1 attB REV2 | This paper | PCR primers | GGGGACCACTTTGTACAAGAAAGCTGGGTGCTACTTCGCTGTCATCATTTGTACAAACTC; Made by IDT. |
| Peptide, recombinant protein | α-bungarotoxin | Tocris | 2133 | |
| Chemical compound, drug | Isradipine; Israd |
Sigma-Aldrich | I6658 | |
| Chemical compound, drug | BayK 8644; BayK | Sigma-Aldrich | B133 | |
| Chemical compound, drug | Ru360 | Millipore | 557440 | |
| Chemical compound, drug | NAD+ | Sigma-Aldrich | N1511 | |
| Chemical compound, drug | NADH | Cayman Chemical | 16038 | |
| Chemical compound, drug | Tricaine; MESAB | Sigma-Aldrich | A5040 | |
| Chemical compound, drug | Paraformaldehyde: EM | Electron Microscopy Sciences | 15710 | |
| Chemical compound, drug | Glutaraldehyde: EM | Electron Microscopy Sciences | 16210 | |
| Chemical compound, drug | Paraformaldehyde: IHC | ThermoFisher Scientific | 28906 | |
| Chemical compound, drug | TRO 19622; TRO |
Cayman Chemical | 21264 | Vortex vigorously to dissolve in embryo media + 0.1% DMSO |
| Software, algorithm | Prism (v. 8) | Graphpad Software | RRID: SCR_002798; https://www.graphpad.com | |
| Software, algorithm | Adobe Illustrator | Adobe | RRID: SCR_014198; https://www.adobe.com | |
| Software, algorithm | FIJI is just ImageJ | NIH | RRID: SCR_003070; https://fiji.sc | |
| Software, algorithm | Zen | Zeiss | RRID: SCR_01367; https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html | |
| Software, algorithm | Prairie View | Bruker Corporation | RRID: SCR_017142; https://www.bruker.com/products/fluorescence-microscopes/ultima-multiphoton-microscopy/ultima-in-vitro/overview.html | |
| Software, algorithm | Python Programming Language | Python Software Foundation | RRID: SCR_008394; https://www.python.org/ |