FIG 1.

Effect of LT gene deletion on cell growth and biofilm formation of S. aureus MR23. (A) Cell growth of wild-type (WT) and LT mutants. The average of three independent cultures is shown. (B) Western blot showing levels of IsaA and SceD in culture supernatants of WT and LT mutants. (C) Biofilm formation of WT and LT mutants without (white bars) or with (gray bars) plasmid. Biofilms formed on the bottom of the 96-well plate were stained with crystal violet; subsequently, absorbance at 595 nm was measured. Representative images of stained biofilms are shown below. The values are the means of six independent cultures. The error bars indicate the standard error of the mean. Differences between WT and mutants were compared using Student’s t test. **P < 0.01, ***P < 0.001. EV, pCN51Cm (empty vector); pCNisaA, pCN51Cm containing isaA; pCNsceD, pCN51Cm containing sceD.