FIG 2.

Effect of LT gene deletion on PBP2a production in S. aureus MR23. (A and B) Western blot showing levels of PBP2a in wild-type (WT) and LT mutants in the presence (A) and absence (B) of plasmids. (C) Western blot showing levels of PBP2a in mecA and isaA mutants. Concentrations of oxacillin and CdCl₂ used for induction are indicated above the panels. As loading controls, CBB-stained gels of the same area are shown below the panels. EV, pCN51Cm (empty vector); pCNisaA, pCN51Cm containing isaA; pCNmecA, pCN51Cm containing mecA.